Raw Sequencing Data Upload

MicrobiomeAnalyst expects demultiplexed, per-sample, compressed sequence files together with a metadata file describing the sample information. It supports either single or paired-end raw 16s sequencing data. The implementation is based on the DADA2 pipeline. Both raw data files and meta-data below are Required.

  1. Sequencing data uploaded as individual zip/fastq.gz files - one zip per data [max: 100 files].
  2. Metadata uploaded as a plain text (.txt) file containing multiple columns - files names, group labels and other experiment factors [example]

Please Select all files, then click Upload to start. Once the upload has completed, click Proceed to continue.


Try our example data

A demo example dataset containing 10 fastq files.
An example dataset containing 12 ITS fastq files.
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