Raw Sequencing Data Upload

MicrobiomeAnalyst expects demultiplexed, compressed sequence files together with a metadata file describing the samples. It supports single or paired-end 16S/18S/ITS amplicon sequencing data based on the DADA2 pipeline.

  1. Sequencing data uploaded as individual zip/fastq.gz files - one zip per data (no larger than 100 MB) [max: 100 files].
  2. The original fastq files (before compress) must end with R1.fastq/R2.fastq. The names of the.fastq files should be consistent before and after compression.
  3. Metadata uploaded as a plain text (.txt) file containing multiple columns - files names, group labels and other experiment factors [example]

Please Select all files, then click Upload to start. Once the upload has completed, click Proceed to continue.

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Try our example data
A demo example dataset containing 10 fastq files.
An example dataset containing 12 ITS fastq files.